PDF KAPA Library Quantification Technical Guide DNA Sequencing Template Preparation HTML Primer & Template Submission Preparation HTML Genotyping Sample Quality HTML Bioanalyzer Sample Submission for RNA Analysis HTML Zeiss Confocal Microscope Confocal Registration Forms PDF Confocal Usage Policy & Instrument Care HTML Illumina HTS Manuals and Documents (as of November 2014) To request any of the below documents, please contact Mark Dasenko . Quantification Data Analysis Excel .xlsx - KAPA Library... DNA and template material suitable for PCR-based processes such as library amplification, qPCR-based library quantification, cluster amplification and sequencing. Both scenarios result in suboptimal sequencing capacity. Data Analysis Template v4.14 This spreadsheet is designed for the analysis of NGS library quantification data generated with the KAPA Library Quantification Kit for Illumina® platforms. A notable improvement in quantitation consistency was observed for concentrations determined by . Library quantification was done using the KAPA library quantification kit (KK4824; Kapa Biosystems) to ensure the final library concentration prior to normalization and pooling for sequencing (40). KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification. Program your thermocycler according to the instrument guidelines. The final libraries are quantified by qPCR assay using KAPA library quantification kit (cat.# MM9 genome files were used in our analysis for the paper. PDF Procedure & Checklist - Full-Length 16S Amplification ... - UC Davis The workflow employs 2 rounds of PCR, the first with universal primer-tailed 16S primers and the second with PacBio Barcoded Universal Primers. Triplicate results for qPCR are important for . $ 292.00. Kapa Biosystems - ScienceVision Library Quantification and Quality Control Quick Reference Guide

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